Questions: 1. Briefly explain how HPLC is used as a separation technique. 2. What is the purpose of the mobile phase? Of the stationary phase? 3. What is the purpose of the caffeine standards? 4. Why does the syringe have to be carefully rinsed before each use? 5. How could you be certain a peak in the soda was caffeine and not another substance with a similar retention time?

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