Science

QUESTION 3. Describe the characteristics of the beta-catenin staining in the tumour tissue below and its implication with regard to the wnt signalling pathway.

EXPERT ANSWER Besides, β-catenin promotes the progression of tumors via suppressing the T-cell responses . The activity of β-catenin is controlled by a large number of binding partners that affect its stability, cellular localization and transcriptional activity. In the canonical Wnt cascade, β-catenin is the key effector responsible for transduction of the signal to the …

QUESTION 3. Describe the characteristics of the beta-catenin staining in the tumour tissue below and its implication with regard to the wnt signalling pathway. Read More »

A genetic disease causing poor resistance to bacterial infection is linked to an unknown mutation in a gene carried by the X chromosome. The cDNA corresponding to this gene is used as a radioactive probe for Southern blot and Northern blot analyses. The results of these 3 experiments are illustrated below.

Figure: Diagram representing the position (and size) of the DNA fragments (Southern blot, left panel) and RNA transcripts (Northern blot, middle panel) recognized by the radioactive probe. The range of size listed on the left side of the left panel applies only to the Southern blot. The size of the proteins coded by the wild …

A genetic disease causing poor resistance to bacterial infection is linked to an unknown mutation in a gene carried by the X chromosome. The cDNA corresponding to this gene is used as a radioactive probe for Southern blot and Northern blot analyses. The results of these 3 experiments are illustrated below. Read More »

This is the result of the Western blot experiment. Explain the different signals in different lanes in the western blot results. Why did the horse serum give the weakest signal?

These antibodies were used: Rabbit anti-rat serum primary antibody binding Goat anti-rabbit IgG conjugated with alkaline phosphatase (AP) as secondary antibody EXPERT ANSWER As per mentioned experiments, have to write different molecular weight of protein marker. In lane 2, lane 3, lane 4, lane 5, lane 7, lane 8, lane 9 the molecular weight of the given …

This is the result of the Western blot experiment. Explain the different signals in different lanes in the western blot results. Why did the horse serum give the weakest signal? Read More »

name the various cell components involved with protein synthesis. How does each participate?

EXPERT ANSWER Answer : Various cell components involved in protein synthesis : Nucleus → Ribosome → Endoplasmic Reticulum → Golgi Apparatus. 1. Nucleus: Gene Transcription (DNA to messenger RNA (mRNA)). 2. Ribosome: mRNA translated to a polypeptide (for proteins staying in the cell, this is the last stop. For proteins to be secreted out of …

name the various cell components involved with protein synthesis. How does each participate? Read More »

Which of the following are examples of sexual selection? (Check all that apply)

a. The male peacock tail display b. The evolution of hemoglobin in response to altitude c. The fighting mandibles of the stag beetle d. The bright colors of males in many species of birds e. The large sexual size dimorphism in elephant seals f. The evolution of warning coloration in poisonous snakes (such as the …

Which of the following are examples of sexual selection? (Check all that apply) Read More »